SubtiBank SubtiBank
Version comparison:

2017-04-24 15:34:12Tue Dec 13 2016 11:16:30 GMT+0100 (CET)

proteinLength

154

geneLength

462

Gene

Coordinates

101,449 → 101,913

The protein

Modification

phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [[protein|ClpC]]-[[protein|ClpP]]) [Pubmed|27749819]

phosphorylation of a tyrosine residue by [[protein|McsB]] [Pubmed|14984053]

recently, it was reported that CtsR is phosphorylated by [[protein|McsB]] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]

in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]

phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [SW|ClpC]-[SW|ClpP]) [Pubmed|27749819]

phosphorylation of a tyrosine residue by [[protein|McsB]] [Pubmed|14984053]

recently, it was reported that CtsR is phosphorylated by [[protein|McsB]] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]

in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]

Expression and Regulation

Operons

[[this]]

Expression and Regulation

Other regulations

[[this]]

Biological materials

Mutant

MGNA-B928 (yacG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1927 NBRP B. subtilis, Japan]

''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab

BKE00830 (''[[gene|ctsR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)

MGNA-B928 (yacG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1927 NBRP B. subtilis, Japan]

''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab

BKE00830 (''[[protein|ctsR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)

Biological materials

two-hybrid system

''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

[SW|Categories] containing this gene/protein

[SW|proteolysis], [SW|transcription factors and their control], [SW|general stress proteins (controlled by SigB)], [SW|heat shock proteins], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence)

101,449 -> 101,913

The protein

[SW|Interactions]

[[protein|McsA]]-[[protein|McsB]]-[[protein|CtsR]]

[[protein|CtsR]]-[[protein|ClpP]]/[[protein|ClpC]] (degradation of [[protein|CtsR]]) [Pubmed|11179229,16163393,17380125]

Expression and Regulation

Operon

''[[protein|ctsR]]-[[protein|mcsA]]-[[protein|mcsB]]-[[protein|clpC]]-[[protein|radA]]-[[protein|disA]]'' [Pubmed|8793870]

Expression and Regulation

[SW|Sigma factor]

[[protein|SigA]] [Pubmed|8793870], [[protein|SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]

Expression and Regulation

Regulation

induced by stress ([[protein|SigB]]) [Pubmed|11544224]

repressed at low temperatures ([[protein|CtsR]]) [Pubmed|9987115]

Expression and Regulation

Regulatory mechanism

[[protein|CtsR]]: transcription repression [Pubmed|9987115]

Expression and Regulation

Additional information

the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]